2/6-2/7/2017 “Management of Large CryoEM Facilities”
A Workshop focused on the management of multi-user cryoEM facilities was held on February 6-7, 2017. The objective was to discuss the infrastructure and operations needed to run a large CryoEM facility. Topics of discussion included everything from the basic facilities. computational infrastructure, workflow and software pipelines to how to manage users.
We thank Thermo Fisher/FEI for generous financial support for this workshop.
The workshop was recorded and can be found at the NRAMM/SEMC Youtube channel: https://www.youtube.com/channel/UCgd1dW4HQ_yvpRjFc670htw
The slides shows in the talks are available as pdf documents – click on the lecturer’s names in the agenda below to get the pdf document.
Workshop on Management of Large CryoEM Facilities
February 6-7, 2017
Monday February 6th
9:00 am Introduction Clint Potter
9:45 am User base Bram Koster; Alistair Siebert; Ed Eng
How are users selected/ deselected? Who are your users (EM, X-ray, other)? What is experience level? What do the users want to do? Just collect data or do they need help from specimen preparation through processing and interpretation. Do users need to come to your facility or is remote access possible? Do users pay for microscope time or for other use of the facility?
10:45 am Break
11:00 am Sample Handling Zhiheng Yu; Scott Stagg; Giovanna Scapin
Sample and grid storage. How do you store, label and keep track of samples and grids? How many samples and grids are handled in a month?
1:00 pm Microscopy Hong Zhou; Wim Hagen
How do users use the microscope? How are users scheduled? Data collection strategies (software, protocols). Are sessions aborted if samples are not good? How is throughput measured? What is throughput?
2:00 pm Processing Ed Eng; Alun Ashton
What packages are being used? Who does the processing? What support (hardware, software) is provided to users?
3:00 pm Break
3:30 pm Workflow Hong Zhou; Zhiheng Yu
What is workflow from sample to map? What data is delivered to the end user? How are projects tracked? How is data organized?
4:30 pm Monitoring and Proactive Maintenance Raymond Schrijver
“Accelerate cryoEM success by remote system monitoring, user training, and support for facility service model”.
Tuesday February 7th
9:00 am Computational Environment Scott Stagg
Hardware and software? What is data/computational flow from microscope to user? What data does user get? How is it delivered? Is data archived? How?
10:00 am Training Bram Koster; Ed Eng
How are users trained? What level of training is given? Who does the training?
11:00 am Break
11:30 am Panel: How do you measure success?
12:30 pm Lunch
1:30 pm Wrap up: Discussion on Best Practices
3:00 pm End
For all further inquiries send email to: email@example.com
Clint Potter and Bridget Carragher
National Resource for Automated Molecular Microscopy
Simons Electron Microscopy Center
New York Structural Biology Center, New York, NY