2012 Workshop Lectures
A Workshop on Advanced Topics in EM Structure Determination: Optimization and Validation
November 11-16, 2012
The PDF files of the lectures presented during the course are reproduced here with the kind permission of the individual lecturers. This material is provided for educational use only. The information in these slides including all data, images and related materials are the property of the individual lecturer. No part of this material may be reproduced without explicit written permission from the lecturer.
| Day 1: Sunday November 11 |
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| Title | Speaker | Audio | Slides |
| Welcome | Bridget Carragher | ||
| The Biological Challenges | Tom Walz |  |
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| The Technical Challenges | Niko Grigorieff | |
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| Day 2: Monday November 12 |
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| Title | Speaker | Audio | Slides |
| Introduction to domain/subunit identification and labeling strategies | David DeRosier | |
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| Optimizing specimen preparation | Holger Stark | |
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| Fab labeling as a strategy for small molecules | Yifan Cheng | |
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| Spotiton: A new approach to EM specimen preparation | Tilak Jain | |
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| DOLORS: Versatile Strategy for Internal Labeling and Domain Localization in Electron Microscopy | Ian MacRae | |
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| New innovations for capturing macromolecules | Debbie Kelly | |
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| Panel Discussion | David DeRosier (Chair) | |
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| Opening windows into the cell: Focused ion beam micromachining of eukaryotic cells for cryo-electron tomography. | Elizabeth Villa | |
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| Day 3: Tuesday November 13 |
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| Title | Speaker | Audio | Slides |
| Introduction and new approaches | Wah Chiu | |
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| Optimizing image acquisition | John Rubinstein | |
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| Direct Detectors Forum: Short contributions from people who have real life experience with these instruments. | David Agard (Discussion Leader)Yifan Cheng (K2),
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| Additional High-end Instrumentation Forum | Bob Glaeser, Discussion LeaderAlternatives for General Access to High-end Equipment
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| Furthering our understanding of microtubule dynamic instability by cryoEM | Gabe Lander | |
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| Day 4: Wednesday November 14 |
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| Title | Speaker | Audio | Slides |
| Introduction and new approaches | Pawel Penczek | |
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| Optimizing image processing | Sjors Scheres | |
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| Single-Particle Electron Microscopy Reveals Extensive Conformational Variability of the Ltn1 E3 Ligase | Dmitry Lyumkis | |
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| Day 5: Thursday November 15 |
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| Title | Speaker | Audio | Slides |
| Introduction | Richard Henderson | |
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| A case study for validation | Steve Ludtke | |
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| Cryo-EM Study of Type II Chaperonin Mm-Cpn in Recognition with human γD-crystallin | Bo Chen | |
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| Day 6: Friday November 16 |
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| Title | Speaker | Audio | Slides |
| Computational approaches | Andrej Sali | |
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| Probing molecular architecture and interactions using X-ray and EM | Ian Wilson | |
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| Structure of the ATP synthase and respiratory chain complexes in the inner mitochondrial membrane | Werner Kühlbrandt | |
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