2/6-2/7/2017 “Management of Large CryoEM Facilities”

A Workshop focused on the management of  multi-user cryoEM facilities was held on February 6-7, 2017.  The objective was to discuss the infrastructure and operations needed to run a large CryoEM facility. Topics of discussion included everything from the basic facilities. computational infrastructure, workflow and software pipelines to how to manage users.

We thank Thermo Fisher/FEI for generous financial support for this workshop.

The workshop was recorded and can be found at the NRAMM/SEMC Youtube channel: https://www.youtube.com/channel/UCgd1dW4HQ_yvpRjFc670htw

The slides shows in the talks are  available as pdf documents – click on the lecturer’s names in  the agenda below to get the pdf document.

Final Agenda:

Workshop on Management of Large CryoEM Facilities
February 6-7, 2017
Monday February 6th

9:00 am    Introduction            Clint Potter

9:45 am    User base       Bram Koster; Alistair Siebert; Ed Eng    
How are users selected/ deselected? Who are your users (EM, X-ray, other)? What is experience level? What do the users want to do? Just collect data or do they need help from specimen preparation through processing and interpretation. Do users need to come to your facility or is remote access possible? Do users pay for microscope time or for other use of the facility?
10:45 am     Break

11:00 am    Sample Handling        Zhiheng Yu; Scott Stagg; Giovanna Scapin 
Sample and grid storage. How do you store, label and keep track of samples and grids? How many samples and grids are handled in a month?

12:00         Lunch

1:00 pm     Microscopy            Hong Zhou; Wim Hagen
How do users use the microscope?  How are users scheduled? Data collection strategies (software, protocols). Are sessions aborted if samples are not good?  How is throughput measured? What is throughput?

2:00 pm     Processing            Ed Eng; Alun Ashton
What packages are being used? Who does the processing? What support (hardware, software) is provided to users?

3:00 pm    Break

3:30 pm     Workflow            Hong Zhou; Zhiheng Yu
What is workflow from sample to map? What data is delivered to the end user? How are projects tracked? How is data organized?

4:30 pm    Monitoring and Proactive Maintenance    Raymond Schrijver 
“Accelerate cryoEM success by remote system monitoring, user training, and support for facility service model”.

Tuesday February 7th

9:00 am     Computational Environment     Scott Stagg
Hardware and software? What is data/computational flow from microscope to user? What data does user get?  How is it delivered?  Is data archived?  How?

10:00 am    Training            Bram Koster; Ed Eng
How are users trained? What level of training is given? Who does the training?

11:00 am    Break

11:30  am    Panel:  How do you measure success?

12:30  pm    Lunch

1:30 pm    Wrap up:  Discussion on Best Practices

3:00 pm    End

For all further inquiries send email to: cpotter@nycbc.org


Clint Potter and Bridget Carragher

National Resource for Automated Molecular Microscopy

Simons Electron Microscopy Center

New York Structural Biology Center, New York, NY