2014 Workshop Lectures
Workshop on Advanced Topics in EM Structure Determination: Where do we go from here?
November 9-14, 2014
The PDF files of the lectures presented during the course are reproduced here with the kind permission of the individual lecturers. This material is provided for educational use only. The information in these slides including all data, images and related materials are the property of the individual lecturer. No part of this material may be reproduced without explicit written permission from the lecturer.
| Day 1: Sunday November 9 |
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| Title | Speaker | Audio | Slides |
| Welcome | Bridget Carragher, Ron Milligan | ||
| Challenges for Molecular Structure Determination | Yifan Cheng |  |
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| Challenges for Cellular Structure Determination | John Briggs | |
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| Day 2: Monday November 10 | |||
| Title | Speaker | Audio | Slides |
| Challenges remaining for specimen preparation | Tom Walz | |
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| New substrates | Lori Passmore | |
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| Spotiton/Typhon | Clint Potter | |
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| Single cell imaging | Henning Stahlberg | |
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| FIB milling | Elizabeth Villa | |
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| New NIH announcement for support of regional consortia for cryoEM data collection | Paula Flicker | |
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| Panel Discussion | David DeRosier (Chair) | |
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| Structural studies of an AAA+ ATPase by cryo electron microscopy | Minglei Zhao | |
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| Day 3: Tuesday November 11 | |||
| Title | Speaker | Audio | Slides |
| Optimizing image acquisition | John Rubinstein | |
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| Even higher resolution? | Holger Stark | |
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| Even better cameras? | Greg McMullan | |
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| Direct Detectors Forum: David Agard (Discussion Leader) |
Yifan Cheng, Wah Chiu, Carsten Sachse, Sjors Scheres |
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| Phase plates for single particles | Rado Danev | |
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| Phase plates for tomography | Wah Chiu | |
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| Panel Discussion | Bob Glaeser (Chair) | |
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| Day 4: Wednesday November 12 |
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| Title | Speaker | Audio | Slides |
| Introduction and new approaches | Sjors Scheres | |
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| New challenges for processing heterogeneity | Niko Grigorieff | |
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| Software and processing challenges | Steve Ludtke | |
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| Energy landscape analysis | Joachim Frank | |
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| Panel on mathematical and computational issues in cryo-EM reconstruction | Chair: Leslie Greengard | |
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| Day 5: Thursday November 13 |
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| Title | Speaker | Audio | Slides |
| Validation | Peter Rosenthal | |
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| New approaches to validation | Pawel Penczek | |
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| Chasing the chain | Frank DiMaio | |
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| Near-atomic resolution cryoEM: how far can we go? | David Veesler/Melody Campbell | |
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| Panel Discussion: Where do we go from here? | Gabriel Lander (Chair) | |
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| FEI Breakout Session | Matthijn Vos | |
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| Processing Movies from Direct Electron Detection Cameras | Ben Bammes | |
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| Model building, fitting & validation using high-resolution cryo-EM maps | Doreen Matthies | |
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| Day 6: Friday November 14 | |||
| Title | Speaker | Audio | Slides |
| How to choose the optimal microscope/camera combinations | John Rubenstein, Henning Stahlberg |
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| The essentials of a cryoEM lab | Justin Kollman, Melanie Ohi, Yifan Cheng |
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| Computational infrastructure | Holger Stark, Steve Ludtke, Daniel Southworth |
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