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Welcome to NRAMM

The National Resource for Automated Molecular Microscopy (NRAMM) is a NIGMS Biomedical Technology Research Resource based at the Simons Electron Microscopy Center, New York Structural Biology Center. Our mission is to develop, test and apply technology aimed towards completely automating the processes involved in solving macromolecular structure using cryo-electron microscopy (cryoEM). Our goal is to both serve as a center for high-throughput molecular imaging and to transfer this technology to the research community.

NRAMM activities include: Technology Research and Development; Support for Driving Biological and Collaborative and Service Projects, Training and Dissemination. We welcome applications from new Collaborative and Service Projects with a need for NRAMM resources and interested users can apply here.

News and Events

Workshop on Smart Data Collection for CryoEM

Workshop on Smart Data Collection for CryoEMOver the past 20 years, data collection for cryoEM has become highly automated. However, most data collection sessions still require operator oversight to obtain the highest quality data. This goal of this workshop was to address next generation data collection strategies that integrate machine learning and real-time processing to reduce …Read more...

Holey Gold Grid Making Workshop

Holey Gold Grid Making WorkshopA practical workshop, which provides an overview of making holey gold foils from holey carbon foils supported on gold mesh grids. “Holey gold” grids provide a stronger support film and reduce beam induced movement of the specimen compared with traditional substrates. For more information see the workshop materials from the February 14, …Read more...

Combination of cryo-EM and genetics to study eukaryotic cilia

Combination of cryo-EM and genetics to study eukaryotic ciliaMasahide Kikkawa, Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo12PM – December 10, 2019 Eukaryotic cilia are complex cell organelles and play important roles in various cell, such as propeller and antenna. They consist of hundreds of different proteins that are precisely organized by self-assembly mechanisms. To …Read more...

Workshops
Appion Part 3 – TomographyRead more...

VitroJet: Bridging the Gap in Sample Prep

VitroJet: Bridging the Gap in Sample PrepGiulia Weissenberger, MScApplication Engineer, CryoSol World12:00 PM Tuesday, October 15 Through many exciting developments, cryo-electron microscopy (EM) has become a powerful method to elucidate 3D structures of macromolecular complexes. This knowledge provides valuable insight in understanding their functions and is the foundation for developing novel medicines. With the increasing demand of cryo-EM, …Read more...

Electron Cryotomography Provides Evidence of a Novel Pathway for Tubulin’s Polymerization into Microtubules

Electron Cryotomography Provides Evidence of a Novel Pathway for Tubulin’s Polymerization into MicrotubulesRichard McIntosh, Ph.D.Distinguished Professor EmeritusUniversity of Colorado Boulder, Department of Physics12:00 PM – Friday, October 4, 2019 The McIntosh lab has used electron tomography to examine the tips of microtubules (MTs) growing in vivo and from pure tubulin in vitro. The ends of all these elongating polymers display bent protofilaments …Read more...

Workshops
Appion Part 2: 3D Reconstruction – September 19, 2019This Appion Part 2 Workshop is a follow-on to the Part 1 workshop and focuses on 3D initial models and refinement.Read more...

Appion Part 1 – Basics and 2D Single Particle Analysis

Appion Part 1 – Basics and 2D Single Particle AnalysisThe Appion Part 1 Workshop provides hands-on training for the Appion single-particle data processing workflow. Appion is a “pipeline” for processing and analysis of EM images; it is integrated with Leginon data acquisition, but can also be used stand-alone after uploading images or particle stacks. Topics include analyzing the quality …Read more...