Workshop on Advanced Topics in EM Structure Determination: Challenges and Opportunities
October 29 – November 3, 2017
The PDF files of the lectures presented during the course are reproduced here with the kind permission of the individual lecturers. This material is provided for educational use only. The information in these slides including all data, images and related materials are the property of the individual lecturer. No part of this material may be reproduced without explicit written permission from the lecturer.
Day 1: Sunday October 29
Title
Speaker
Audio
Slides
Welcome
Bridget Carragher, Clint Potter
Challenges and Opportunities for Molecular Structure Determination
Yifan Cheng
Challenges and Opportunities for Cellular Structure Determination
Elizabeth Villa
Day 2: Monday October 30
Title
Speaker
Audio
Slides
Challenges remaining for specimen preparation
Bob Glaeser
New approaches to substrates and specimen preparation
Chris Russo
Spotiton and nanowire grids
Bridget Carragher
Microfluidic Sample Preparation: Opportunities, Challenges and Visual Proteomics
Thomas Braun
CryoET as a method to understand the air-water interface
Alex Noble
Time resolved cryoEM
Jack Fu
High throughput cryo-ET: Visualizing molecular machines in action
Jun Liu
Day 3: Tuesday October 31
Title
Speaker
Audio
Slides
Optimizing image acquisition
John Rubinstein
Phase plates
Rado Danev
Next generation cameras
Scott Stagg (DE64),
Paul Mooney (K3),
Chris Russo
Day 4: Wednesday November 1
Title
Speaker
Audio
Slides
Introduction and new approaches
Sjors Scheres
New challenges
Niko Grigorieff
Deep learning methods
Steve Ludtke
Bayesian methods in cryo-EM
Marcus Brubaker
Software tools to deploy and manage cryo-EM jobs in the cloud
Michael Cianfrocco
CFTR, the odd ABC transporter responsible for cystic fibrosis
Jue Chen
Day 5: Thursday November 2
Title
Speaker
Audio
Slides
Challenges and Opportunities: SP
Wah Chiu
Challenges and Opportunities: CryoET
Julia Mahamid
Fitting challenges
David Veesler
Molecular machines in action
Thomas Marlovits
Poster Talks
Alex Brilot,
Jessica Rabuck-Gibbons,
William Rice
Day 6: Friday November 3
Title
Speaker
Audio
Slides
How to choose the optimal microscope/camera combinations
Anchi Cheng
Exploring the Size and Resolution Limits of Single Particle Cryo-electron Microscopy at 200 keV