2017 Workshop Lectures

Workshop on Advanced Topics in EM Structure Determination: Challenges and Opportunities

October 29 – November 3, 2017

The PDF files of the lectures presented during the course are reproduced here with the kind permission of the individual lecturers. This material is provided for educational use only. The information in these slides including all data, images and related materials are the property of the individual lecturer. No part of this material may be reproduced without explicit written permission from the lecturer.

Day 1: Sunday October 29
Title Speaker Audio  Slides
Welcome Bridget Carragher, Clint Potter  
Challenges and Opportunities for Molecular Structure Determination Yifan Cheng
Challenges and Opportunities for Cellular Structure Determination Elizabeth Villa
Day 2: Monday October 30
Title Speaker Audio Slides
Challenges remaining for specimen preparation Bob Glaeser
New approaches to substrates and specimen preparation Chris Russo
Spotiton and nanowire grids Bridget Carragher
Microfluidic Sample Preparation: Opportunities, Challenges and Visual Proteomics Thomas Braun
CryoET as a method to understand the air-water interface Alex Noble
Time resolved cryoEM Jack Fu
High throughput cryo-ET: Visualizing molecular machines in action Jun Liu
Day 3: Tuesday October 31
Title Speaker Audio Slides
Optimizing image acquisition John Rubinstein
Phase plates Rado Danev
Next generation cameras Scott Stagg (DE64),
Paul Mooney (K3),
Chris Russo

Day 4: Wednesday November 1
Title Speaker Audio Slides
Introduction and new approaches Sjors Scheres
New challenges Niko Grigorieff
Deep learning methods Steve Ludtke
Bayesian methods in cryo-EM Marcus Brubaker
Software tools to deploy and manage cryo-EM jobs in the cloud Michael Cianfrocco
CFTR, the odd ABC transporter responsible for cystic fibrosis Jue Chen  
Day 5: Thursday November 2
Title Speaker Audio Slides
Challenges and Opportunities: SP Wah Chiu
Challenges and Opportunities: CryoET Julia Mahamid  
Fitting challenges David Veesler
Molecular machines in action Thomas Marlovits
Poster Talks Alex Brilot,
Jessica Rabuck-Gibbons,
William Rice

Day 6: Friday November 3
Title Speaker Audio Slides
How to choose the optimal microscope/camera combinations Anchi Cheng  
Exploring the Size and Resolution Limits of Single Particle Cryo-electron Microscopy at 200 keV Mark Herzik
The essentials of a cryoEM lab David Veesler,
Joaquin Ortega

Computational Infrastructure Ed Eng
Computational Considerations Steve Ludtke