2017 Workshop Lectures
Workshop on Advanced Topics in EM Structure Determination: Challenges and Opportunities
October 29 – November 3, 2017
The PDF files of the lectures presented during the course are reproduced here with the kind permission of the individual lecturers. This material is provided for educational use only. The information in these slides including all data, images and related materials are the property of the individual lecturer. No part of this material may be reproduced without explicit written permission from the lecturer.
| Day 1: Sunday October 29 |
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| Title | Speaker | Audio | Slides |
| Welcome | Bridget Carragher, Clint Potter |  |
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| Challenges and Opportunities for Molecular Structure Determination | Yifan Cheng | ||
| Challenges and Opportunities for Cellular Structure Determination | Elizabeth Villa | |
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| Day 2: Monday October 30 |
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| Title | Speaker | Audio | Slides |
| Challenges remaining for specimen preparation | Bob Glaeser | |
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| New approaches to substrates and specimen preparation | Chris Russo | |
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| Spotiton and nanowire grids | Bridget Carragher | |
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| Microfluidic Sample Preparation: Opportunities, Challenges and Visual Proteomics | Thomas Braun | |
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| CryoET as a method to understand the air-water interface | Alex Noble | |
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| Time resolved cryoEM | Jack Fu | |
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| High throughput cryo-ET: Visualizing molecular machines in action | Jun Liu | |
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| Day 3: Tuesday October 31 |
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| Title | Speaker | Audio | Slides |
| Optimizing image acquisition | John Rubinstein | |
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| Phase plates | Rado Danev | |
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| Next generation cameras | Scott Stagg (DE64), Paul Mooney (K3), Chris Russo |
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| Day 4: Wednesday November 1 |
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| Title | Speaker | Audio | Slides |
| Introduction and new approaches | Sjors Scheres | |
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| New challenges | Niko Grigorieff | |
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| Deep learning methods | Steve Ludtke | |
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| Bayesian methods in cryo-EM | Marcus Brubaker | |
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| Software tools to deploy and manage cryo-EM jobs in the cloud | Michael Cianfrocco | |
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| CFTR, the odd ABC transporter responsible for cystic fibrosis | Jue Chen | |
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| Day 5: Thursday November 2 |
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| Title | Speaker | Audio | Slides |
| Challenges and Opportunities: SP | Wah Chiu | |
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| Challenges and Opportunities: CryoET | Julia Mahamid | |
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| Fitting challenges | David Veesler | |
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| Molecular machines in action | Thomas Marlovits | ||
| Poster Talks | Alex Brilot, Jessica Rabuck-Gibbons, William Rice |
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| Day 6: Friday November 3 |
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| Title | Speaker | Audio | Slides |
| How to choose the optimal microscope/camera combinations | Anchi Cheng | |
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| Exploring the Size and Resolution Limits of Single Particle Cryo-electron Microscopy at 200 keV | Mark Herzik | |
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| The essentials of a cryoEM lab | David Veesler, Joaquin Ortega |
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| Computational Infrastructure | Ed Eng | |
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| Computational Considerations | Steve Ludtke | |
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