4/18/2017: Rosetta Workshop – Frank DiMaio

On April 18th, 2017, Dr. Frank DiMaio,of the University of Washington, presented a hands-on tutorial: “Using Rosetta For CryoEM-Guided Protein Structure Determination”. This tutorial introduced several Rosetta-based tools for structure determination guided by cryoEM density data. A general introduction to the Rosetta software suite was provided, and then specific methods for automated de novo structure determination, homology modeling, and model refinement were covered. The tutorial also described interpretation of Rosetta results and introduced several other useful tools in Rosetta to handle other experimental restraints. The workshop was broadcast live and the contents may be viewed on the NRAMM/SEMC Youtube channel at:  https://www.youtube.com/watch?v=IRsObmK9x1A