A Practical Course in Molecular Microscopy
November 02-10, 2005
Center for Integrative Molecular Biosciences (CIMBio)
The Scripps Research Institute (TSRI), La Jolla, California
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This 9 day course is part of the outreach and training activities of the NCRR National Resource for Automated Molecular Microscopy (NRAMM), located at the Center for Integrative Molecular Biosciences (CIMBio) at The Scripps Research Institute (TSRI). The course will include a thorough grounding in all aspects of molecular structural determination by cryo-electron microscopy (cryoEM).
The course will include an extensive exposure to all of the practical aspects of cryoEM as well as a solid grounding in the theory. The basic format will be theoretical lectures in the mornings followed by hands-on lab sessions and demonstrations in the afternoons. The lab sessions will include instruction in specimen preparation, use of the electron microscope for imaging of cryo specimens, evaluation of the images, and a thorough treatment of the image analysis techniques required to reconstruct three dimensional maps. In addition there will be a number of afternoon lectures introducing students to research topics in the area of macromolecular structure. Evenings will be reserved for poster presentations by student participants and for helping students with their own projects.
Bridget Carragher, Clinton S. Potter, Ronald A Milligan
National Resource for Automated Molecular Microscopy