Initial Models and Refined Reconstructions

Initial Models and Refined Reconstructions

Initial models and final reconstructions of the 50S ribosomal subcomplex. (A) EMDB ribosomal model showing relative rotations for each view with an emphasis on defining features such as the L1 and L11 protein arms, the central protuberance (CP), and the tRNA cleft with the protein transferase center (PTC). (B-F) Rotated views of the ribosomal subunit are shown for each initial model (left) and final reconstruction (right). The best resolution is given by FSC0.5 and RMeasure, respectively. All initial models refined to identical final reconstructions.

Lyumkis D, Voss NR – Cheng A, Lau PW, Mulder A, Lander GC, Brignole EJ, Fellmann D, Irving C, Jacovetty EL, Leung A, Pulokas J, Quispe JD, Winkler H, Yoshioka C, Carragher B, Potter CS