Cryoelectron Microscopy of Keyhole Limpet Hemocyanin using Leginon
a. Image of a detected hole at intermediate magnification. b. Example of an image acquired in the hole at high magnification. Images a and b were acquired directly on a digital camera. c. FFT of the focus image. d. Surface representation of the 3D reconstruction volume.
Images courtesy of NRAMM, Fabrice Mouche