CCMV Reconstruction in 8 hours

CCMV Reconstruction in 8 hours

On Nov. 13, 2006, we showcased the power of the Leginon pipeline by reconstructing CCMV to 12 Angstroms within 8 hours with minimal human interaction. The schedule went as follows:
7:00 AM – Frozen CCMV grid enters the microscope, and Leginon is set up.
9:30 AM – Leginon begins automatically collecting images at 80,000 X magnification. Particles are selected automatically with Selexon & CTF is estimated automatically with ACE, all concurrently with data collection. This information is all stored in the database.
Noon – An image stack containing 8342 particles is created from the micrographs & a refinement started on the “Garibaldi” cluster using 40 processors. A 20 Angstrom EM reconstruction from Tim Baker’s lab is used as a starting model.
3PM – A 12 Angstrom reconstruction of CCMV is complete.

Gabriel Lander – AMI