Automated Image Acquisition, Particle Detection and Classification for Cowpea Mosaic Virus (CPMV)

Automated Image Acquisition, Particle Detection and Classification for Cowpea Mosaic Virus (CPMV)

In an single experiment session, a total of 1584 high magnification negative-stained images (62000x, -600nm defocus) of cowpea mosaic virus (CPMV) were acquired using Leginon. A total of 36619 particles were automatically detected and then classified into three classes: full capsid (23277), empty capsid (8678) and junk (4664). An example image outlined with automatically targeted CPMV particles is shown to the left where a red “+” indicates a targeted full capsid particle and a blue “+” an empty capsid particle. Image courtesy of NRAMM and Wendy F. Ochoa from TSRI.

Wendy F. Ochoa – NRAMM