Wednesday September 16, 2015
“Automated Fiducial-less Tilt-Series Alignment with Protomo. ”
Alex Noble, Florida State University
For more than a decade, tilt-series collection using gold fiducial beads has allowed for structural determination of in situ cellular structures as well as heterogeneous macromolecular structures. However, not all specimen are amenable to the use of fiducials. The emergence of direct electron detectors has enabled the collection of low dose, high contrast images, opening the door for reliable correlation-based tilt-series alignment. I will present a set of automated, correlation-based tilt-series alignment, CTF correction, and reconstruction workflows from within Appion with the goal of making the production of high quality tomograms accessible to all users.