Using NRAMM

Overview

The mission at NRAMM is to develop cryoEM as a mainstream and efficient methodology on a par with, and complementary to, X-ray crystallography and other equivalent hybrid structural methods. In addition, we see a role for structural EM as being uniquely capable of providing information about the dynamics of molecular machines. Technology Research and Development (TRD) efforts in the current funding cycle are focused in 3 areas: TRD#1: CryoEM Specimen Preparation Using Piezo-based Picoliter Dispensing; TRD#2: Optimizing Performance, Resolution, and Throughput of CryoTEM; TRD#3: Appion: An enabling tool for EM processing and analysis of complex systems.

The TRD activities at NRAMM are driven by close interactions and feedback from Driving Biological projects (DBPs) and further tested and validated by  Collaborative and Service Projects (CSPs). We welcome applications from new Collaborative and Service Projects with a need for NRAMM resources and interested users can apply using the form below. CSP projects accepted by NRAMM will have access to the advanced technology developed at NRAMM (described further in the links to the TRD projects above) as well as the instrumentation available at NRAMM, including  Titan Krios microscopes equipped with direct detectors, phase plates and energy filters.  More details on instrumentation are available on the infrastructure pages.

Data Access for Current CSP Projects

CSP projects that have used the facilities at NRAMM to acquire data can view their data by loging in  here.

Application for New CSP Projects

NRAMM welcomes applications of both collaborative and service projects.

Users applying to the Resource are asked to proceeed as follows:

    1. Registration: Register an account.
    2. Project Submission: Submit a Project. (If you already have an account with us)
    3. Review and Selection: Your application will be reviewed by the resource executive committee (REC) with one month. Selection of projects is based primarily on scientific value and synergy with the core research and development projects. Additional consideration will be given to geographical location and inclusion of underrepresented communities.
    4. Project Operation: Once the project has been accepted it will be tracked on a weekly basis. An initial interaction between the new users and the Resource staff will take place and a plan will be developed for the project.
    5. Scheduling of Facility Resources: The REC will oversee the allocation of instrumentation and staff resources. Instrumentation is scheduled using a web based calendar system.

Users of the Resource are requested to acknowledge use of the Resource in publications as follows:
 “Some of the work presented here was conducted at the National Resource for Automated Molecular Microscopy located at the New York Structural Biology Center, supported by grants from the NIH National Institute of General Medical Sciences (GM103310) and the Simons Foundation (SF349247).”

We also welcome the opportunity to develop new DBPs and interested researchers are encouraged to contact us directly at: sramm@nysbc.org