November 11-16, 2012: A Workshop on Advanced Topics in EM Structure Determination: Optimization and Validation

A Workshop on Advanced Topics in EM Structure Determination: Optimization and Validation

November 11-16, 2012

A major goal of the workshop was  to discuss some of the challenges that we face in using cryoEM to solve the structure of macromolecules.  Topics discussed  included improvements in specimen preparation, imaging, processing and reconstruction, and methods for validation. The workshop is aimed at students, postdocs and faculty that already have some experience practicing cryoEM. The basic format of the course is  theoretical lectures in the mornings followed by practical lectures and demonstrations and panel discussions in the afternoon. Evenings were reserved for poster presentations by participants and research talks by instructors.

The instructors for the course  included: David Agard (UCSF), Bridget Carragher (TSRI), Yifan Cheng (UCSF), Anchi Cheng (TSRI), Wah Chiu (Baylor), David DeRosier (Brandeis), Joachim Frank (Columbia), Bob Glaeser (LBL), Niko Grigorieff (Brandeis), Richard Henderson (MRC), Tilak Jain (TSRI), Debbie Kelly (Virigina Tech.), Werner Kuhlbrandt (MPI), Gabe Lander (Berkeley), Steve Ludtke (Baylor), Ron Milligan (TSRI), Pawel Penczek (U. Texas), Clint Potter (TSRI), John Rubinstein (Toronto), Andrej Sali (UCSF), Sjors Scheres (MRC), Holger Stark (MPI), Elizabeth Villa (MPI), Tom Walz (Harvard), Ian WIlson (TSRI).

The  final agenda can be viewed here.

The lectures in the form of audio recordings and pdf’s of the lecture slides can be found here.