Three-dimensional EM structure of an intact activator-dependent transcription initiation complex

Three-dimensional EM structure of an intact activator-dependent transcription initiation complex

Electron microscopy reconstruction (20 A resolution) of a transcription initiation complex from Escherichia coli comprising RNA polymerase holoenzyme (purple with alpha subunit C-terminal domain shown in green), catabolite activator protein (blue), and DNA (tan). The background is an electron micrograph of reconstituted complex particles in negative stain. Hudson, B., Quispe, J., Lara, S., Kim, Y., Berman, H. M., Arnold, E., Ebright, R. E. & Lawson, C. L. (2009). Proc Natl Acad Sci U S A 106, 19830-19835. Hudson, B., Quispe, J., Lara, S., Kim, Y., Berman, H. M., Arnold, E., Ebright, R. E. & Lawson, C. L. (2009). Proc Natl Acad Sci U S A 106, 19830-19835.

Brian Hudson and Cathy Lawson, Rutgers University – Rutgers University