Forum 11/21/2002

“Automated acquisition using Leginon and three-dimensional reconstruction of the Keyhole Limpet Hemocyanin”

Fabrice Mouche, Research Associate – The Department of Cell Biology, The Scripps Research Insitute, La Jolla, CA

In cryoelectron microscopy, the major difficulty in solving 3D reconstructions to high resolution is the time required to acquire and analyze a large dataset of high-quality images. This time increases dramatically and proportionally to the number of particles required.

To solve these problems, Leginon, a software developed and designed to automatically acquire a large series of high-quality images in ice, was used and applied to a single particle, the Keyhole Limpet hemocyanin.

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