bioRxiv preprint – Spotiton: New Features and Applications

Spotiton: New Features and Applications

Venkata P DandeyHui WeiZhening ZhangYong Zi TanPriyamvada AcharyaEdward T EngWilliam J RicePeter A KahnClinton S. PotterBridget Carragher

We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ~0.3nm resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.