An Integrated Workflow for Cryo-Electron Tomography

Blanca Carbajal González, M.S
Thermo Fisher Scientific
Wednesday April 3, 2019

The ultimate goal of structural and cell biologists is to derive the structures of molecular machines in their natural, functional context.  Cryo-electron tomography is a label-free, fixation-free imaging technique, which provides 3D nanometer-resolution.  However, high resolution cryo-ET has mainly been limited to isolated organelles or the peripheries of cells. With recent advances in thin lamella preparation with the Thermo ScientificTM Aquilos TM Cryo-FIB, researchers are gaining novel insights into previously inaccessible regions of the cell.Here we describe the suite of instruments that enable Thermo Fisher Scientific’s cryo-electron tomography workflow as well as application examples from the recent literature.