4/26/2017: “Addressing Challenges in CryoEM Grid Preparation “

This workshop was held in April at SEMC and addressed challenges in preparing vitrified specimens of suitable ice thickness, and suitable particle preservation, distribution, and orientation, in a repeatable, reliable and robust manner.  A major goal of the workshop was to understand the issues underlying these challenges and discuss new approaches that will lead to improvements in all of these aspects.

We thank TTP Labtech Ltd. for generous financial support for this workshop.

The workshop was webcast and is available on the NRAMM/SEMC channel on Youtube https://www.youtube.com/c/nrammsemc

Final Agenda:

Workshop on Addressing Challenges in CryoEM Grid Preparation
Wednesday, April 26, 2017

9:00 am    Introductions                                                                Clint Potter, Bridget Carragher

9:15 am    What works, what does not                                       Tom Walz    
(20 minute talk, 10 minute discussion)

9:45 am    Member proteins.                                                        John Rubinstein
(20 minute talk, 10 minute discussion)

10:15 am    Using SMALPs to prepare member proteins.      Naomi Pollock
(10 minute talk, 5 minute discussion)

10:30 am    Break

11:00 am    What is wrong with our current picture of         Bob Glaeser
                     cryo-em specimens?
(30 minute talk, 15 minute discussion)

11:45 am    Particle distribution and ice thickness.                Alex Noble
(20 minute talk, 10 minute discussion)

12:15 pm    Tagged proteins and on-grid purification.          Scott Bolton
(10 minute talk, 5 minute discussion)

12:30pm       Lunch

1:30 pm     New Substrates                                                         Chris Russo
(30 minute talk, 15 minute discussion)

2:15 pm     Spotiton and nanowire grids.                                Bridget Carragher, Alex Wei
(30 minute talk, 15 minute discussion)                                   Venkat Dandey

3:00 pm    Grid preparation by spraying/plunging in          Jack Fu
                   time-resolved cryo-EM.
(10 minute talk, 5 minute discussion)

3:15 pm     Towards a time-resolved study                            Radoslav Enchev
(10 minute talk, 5 minute discussion)

3:30 pm     Break

4:00 pm    General Discussion:
                   Is there hope for a standardized protocol for EM?

5:45 pm     Dinner Reception (Maison Harlem, 341 St. Nicolas Avenue, NY 10027)

For all further inquiries send email to: cpotter@nycbc.org

Organizers

Clint Potter and Bridget Carragher

National Resource for Automated Molecular Microscopy

Simons Electron Microscopy Center

New York Structural Biology Center, New York, NY

http://www.nramm.org