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The PDF files of the lectures presented during the course are reproduced
here with the kind permission of the individual lecturers. This material is provided for
educational use only. The information in these slides including all data, images and related
materials are the property of the individual lecturer. No part of this material may be reproduced
without explicit written permission from the lecturer. |
| Day 1: Saturday November 10 | |||
| Title | Speaker | Audio | Slides |
| Introduction/Welcome | Bridget Carragher/ Ron Milligan | |
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| The Future of CryoEM | Richard Henderson | |
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| The Technical Challenges Ahead | David DeRosier | |
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| Day 2: Sunday November 11 | |||
| Title | Speaker | Audio | Slides |
| Specimen Preparation Techniques for High Resolution Structural Studies by Cryo-EM | Yoshi Fujiyoshi | |
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| In-focus Phase Contrast: Expected Improvements, Required Optics, and Research Challenges | Robert Glaeser | |
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| Issues in Image Formation and Acquisition | Richard Henderson | |
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| Cryo Specimen Preparation @NRAMM | Joel Quispe | |
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| Monolayer Purification for SP EM | Deborah Kelly | |
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| Panel: Cryo-preparation and Charging | Ron Milligan, Bob Glaeser, and James Chen | |
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| Electron Crystallograpy of Tubes: Nicotinic Acetylcholine Receptor | Nigel Unwin | |
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| Day 3: Monday November 12 | |||
| Title | Speaker | Audio | Slides |
| Correcting the Image | Niki Grigorieff | |
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| Classification Methods in SP Analysis | Pawel Penczek | |
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| Automated Molecular Microscopy | Bridget Carragher | |
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| Leginon - Part1 | Jim Pulokas and Anchi Cheng | |
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| Leginon - Part2 | Denis Fellmann | |
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| Orthogonal and Random Tilt Reconstruction - Part1 | Andres Leschiziner | |
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| Orthogonal and Random Tilt Reconstruction - Part2 | Craig Yoshioka | |
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| Orthogonal and Random Tilt Reconstruction - Part3 | Neil Voss | |
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| Panel: Approches for Making Specimens and Ordered Arrays | Ron Milligan, Vinzenz Unger, Mark Yeager and Liz Wilson-Kubalek | |
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| Structure of the Aquaporin-0 Membrane Junction | Thomas Walz | |
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| Day 4: Tuesday November 13 | |||
| Title | Speaker | Audio | Slides |
| Single Partical Refinement Strategies: Symmetry Heterogeneity and Dynamics | Steven Ludtke | |
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| Initial Model Generation | Thomas Walz | |
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| Detecting and Sorting Heterogeneity in Macromolecular Assemblies | Eva Nogales | |
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| EMAN2 | Steven Ludtke | |
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| SPARX | Pawel Penczek | |
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| Spider/Spire | Joachim Frank | |
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| Freealign | Niko Grigorieff | |
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| Panel: Instrumentation | Kenneth Downing | |
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| Molecular Tomography | Ken Taylor | |
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| Day 5: Wednesday November 14 | |||
| Title | Speaker | Audio | Slides |
| Conformational Variability: Experience with Ribosomes | Joachim Frank | |
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| Maximum Likelihood Methods | Fred Sigworth | |
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| High Resolution Crystal Structure of the Human Beta2 Adrenergic Receptor | Raymond Stevens | |
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| Iterative Helical Processing | Edward Egelman | |
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| SP Processing Pipeline @ NRAMM | Gabriel Lander and Neil Voss | |
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| Visualization with UCSF Chimera | Thomas Goddard | |
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| Panel: Data Analysis and Modeling | Ron Milligan, Andrew Ward, and Francisco Asturias | |
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| Blending Crystallography and CryoEM to Study STIV: A Virus that Thrives in Bioling Acid | Jack Johnson | |
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| Day 6: Thursday November 15 | |||
| Title | Speaker | Audio | Slides |
| X-ray Methods Applicable to EM | Stephen Harrison | |
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| Fitting and Visualization | Klaus Schulten | |
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| Signature | James Chen | |
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| TOM | Stefan Bohn | |
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| Computer Vision Techniques in EM | Craig Yoshioka | |
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| Implementing 3DEM Standards | David Belnap | |
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| Panel: Matters Arising During the Workshop | Ron Milligan | |
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| Day 7: Sunday November 16 | |||
| Title | Speaker | Audio | Slides |
| Electron Cryotomagraphy: Example Experimental Results that Illustrate how the Technique Can Help Answer Important Biological Questions | Grant Jensen | |
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| CryoEM from Atoms to Organisms | Wah Chiu | |
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